Oxidative Photosynthetic Phosphorylation by Spinach Chloroplasts
نویسندگان
چکیده
منابع مشابه
Oxidative photosynthetic phosphorylation by spinach chloroplasts.
In an earlier note, evidence was presented for the occurrence of a new type of chloroplast-catalyzed photosynthetic phosphorylation called “oxidative photophosphorylation” (I). When spinach chloroplasts are illuminated in the presence of orthophosphate, adenosine diphosphate, and a dye such as trichlorophenol indophenol, adenosine triphosphate is generated provided oxygen is available, but not ...
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In spinach chloroplasts which have been shaken with n-heptane, photophosphorylation coupled to the reduction of nicotinamide adenine dinucleotide phosphate is inhibited about 95% while the photoreduction of NADP+ (and ferricyanide) is stimulated as much as 50%. Photophosphorylation catalyzed by phenazine methosulfate, however, is inhibited only 50%. These data are interpreted to indicate that t...
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The process of photosynthetic phosphorylation by spinach chloroplasts has been under active investigation since it was discovered by Arnon et aZ. (2). There has been some apparent confusion about the effect of oxygen on this process. Thus Arnon et al. (2) first reported that oxygen was necessary for photophosphorylation, but then concluded in a later paper that “in the presence of the proper co...
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The Emerson enhancement effect was evaluated in the intact spinach (Spinacia oleracea var. Long Standing Bloomsdale) chloroplast by monitoring the uptake of (14)CO(2) during illumination by 640 nm and 720 nm lights. Low levels (about 10 mum) of fructose 1,6-diphosphate, ribose 5-phosphate, and glycerate 3-phosphate stimulated the rate of photosynthesis and abolished enhancement values observed ...
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During attempts to prepare active fragments of spinach chloroplasts, we observed major losses of phosphorylative activity following suspension of the chloroplasts in water. This damage was traced to treatments which probably had the effect of removing cations from the chloroplasts, rather than to a simple physical disruption. Indeed, chloroplast fragments have been used successfully in studies ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69891-6